Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases T Mukai, T Kobayashi, N Hino, T Yanagisawa, K Sakamoto, S Yokoyama Biochemical and biophysical research communications 371 (4), 818-822, 2008 | 340 | 2008 |
Codon reassignment in the Escherichia coli genetic code T Mukai, A Hayashi, F Iraha, A Sato, K Ohtake, S Yokoyama, K Sakamoto Nucleic acids research 38 (22), 8188-8195, 2010 | 245 | 2010 |
Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon T Mukai, H Hoshi, K Ohtake, M Takahashi, A Yamaguchi, A Hayashi, ... Scientific reports 5 (1), 9699, 2015 | 167 | 2015 |
Rewriting the genetic code T Mukai, MJ Lajoie, M Englert, D Söll Annual review of microbiology 71, 557-577, 2017 | 155 | 2017 |
Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coli T Mukai, A Yamaguchi, K Ohtake, M Takahashi, A Hayashi, F Iraha, S Kira, ... Nucleic acids research 43 (16), 8111-8122, 2015 | 92 | 2015 |
Genetic-code evolution for protein synthesis with non-natural amino acids T Mukai, T Yanagisawa, K Ohtake, M Wakamori, J Adachi, N Hino, A Sato, ... Biochemical and biophysical research communications 411 (4), 757-761, 2011 | 81 | 2011 |
Facile recoding of selenocysteine in nature T Mukai, M Englert, HJ Tripp, C Miller, NN Ivanova, EM Rubin, ... Angewandte Chemie International Edition 55 (17), 5337-5341, 2016 | 61 | 2016 |
Wide-range protein photo-crosslinking achieved by a genetically encoded N ε-(benzyloxycarbonyl) lysine derivative with a diazirinyl moiety T Yanagisawa, N Hino, F Iraha, T Mukai, K Sakamoto, S Yokoyama Molecular BioSystems 8 (4), 1131-1135, 2012 | 56 | 2012 |
Genetic incorporation of a photo-crosslinkable amino acid reveals novel protein complexes with GRB2 in mammalian cells N Hino, M Oyama, A Sato, T Mukai, F Iraha, A Hayashi, H Kozuka-Hata, ... Journal of molecular biology 406 (2), 343-353, 2011 | 55 | 2011 |
Efficient Decoding of the UAG Triplet as a Full-Fledged Sense Codon Enhances the Growth of a prfA-Deficient Strain of Escherichia coli K Ohtake, A Sato, T Mukai, N Hino, S Yokoyama, K Sakamoto Journal of bacteriology 194 (10), 2606-2613, 2012 | 50 | 2012 |
A facile method for producing selenocysteine‐containing proteins T Mukai, A Sevostyanova, T Suzuki, X Fu, D Söll Angewandte Chemie International Edition 57 (24), 7215-7219, 2018 | 45 | 2018 |
Protein stabilization utilizing a redefined codon K Ohtake, A Yamaguchi, T Mukai, H Kashimura, N Hirano, M Haruki, ... Scientific reports 5 (1), 9762, 2015 | 45 | 2015 |
Multiple Site‐Specific Installations of Nε‐Monomethyl‐L‐Lysine into Histone Proteins by Cell‐Based and Cell‐Free Protein Synthesis T Yanagisawa, M Takahashi, T Mukai, S Sato, M Wakamori, M Shirouzu, ... ChemBioChem 15 (12), 1830-1838, 2014 | 44 | 2014 |
Genetic encoding of non‐natural amino acids in Drosophila melanogaster Schneider 2 cells T Mukai, M Wakiyama, K Sakamoto, S Yokoyama Protein Science 19 (3), 440-448, 2010 | 40 | 2010 |
Transfer RNAs with novel cloverleaf structures T Mukai, O Vargas-Rodriguez, M Englert, HJ Tripp, NN Ivanova, ... Nucleic acids research 45 (5), 2776-2785, 2017 | 34 | 2017 |
Cell-Free Protein Synthesis Using S30 Extracts from Escherichia coli RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins J Adachi, K Katsura, E Seki, C Takemoto, M Shirouzu, T Terada, T Mukai, ... International journal of molecular sciences 20 (3), 492, 2019 | 32 | 2019 |
Engineering an Automaturing Transglutaminase with Enhanced Thermostability by Genetic Code Expansion with Two Codon Reassignments K Ohtake, T Mukai, F Iraha, M Takahashi, K Haruna, M Date, K Yokoyama, ... ACS synthetic biology 7 (9), 2170-2176, 2018 | 29 | 2018 |
RNA-dependent cysteine biosynthesis in bacteria and archaea T Mukai, A Crnković, T Umehara, NN Ivanova, NC Kyrpides, D Söll MBio 8 (3), 10.1128/mbio. 00561-17, 2017 | 22 | 2017 |
Overcoming the Challenges of Megabase-Sized Plasmid Construction in Escherichia coli T Mukai, T Yoneji, K Yamada, H Fujita, S Nara, M Su’etsugu ACS Synthetic Biology 9 (6), 1315-1327, 2020 | 21 | 2020 |
Bioinformatic Analysis Reveals Archaeal tRNATyr and tRNATrp Identities in Bacteria T Mukai, NM Reynolds, A Crnković, D Söll Life 7 (1), 8, 2017 | 21 | 2017 |